Estrogen Induces Different Responses in Dermal and Lung Fibroblasts

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Abstract
Fibroblast-like cells derived from dorsal skin and lung of AKR strain mouse embryos were cultured with estrogen for 4 days. The labeled hydroxyproline content was measured as newly synthesized collagenous protein in the cell layer and media. Although collagen synthesis by both cell lines was increased in both fractions under the influence of .beta.-estradiol-3-benzoate, at physiological concentrations of 10-3-10-1 .mu.g/ml, the rate of increase differed. Fibroblasts derived from skin showed increased collagen synthesis of .apprx. 76%, while those from lung showed an increase of .apprx. 25%. Total protein synthesis by both cell lines also increased. In lung fibroblast cultures the synthesis of total protein was increased more than the synthesis of collagen; in skin fibroblast cultures the synthesis of collagen was increased more than the synthesis of total protein. DNA synthesis by both cell lines was not affected by estrogen at the concentrations used. Fibroblasts apparently develop in a different manner in each organ.