Antigenic specificity of serological response in Chlamydia trachomatis urethritis detected by immunoblotting.

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Abstract
Sera from 19 patients with C. trachomatis culture positive non-gonococcal urethritis were studied for the presence of antibodies to chlamydial proteins by immunoblotting. Ten C. trachomatis negative patients with non-gonococcal urethritis and 10 healthy controls were also studied. Acute phase sera from C. trachomatis positive patients with non-gonococcal urethritis reacted only with the major outer membrane protein whereas all the convalescent phase serum samples reacted with the major outer membrane protein and with a 60,000 and a 62,000 molecular weight protein. Some sera also reacted with a 45,000 molecular weight protein. Five of 10 convalescent phase samples from patients with C. trachomatis negative non-gonococcal urethritis showed a reaction pattern comparable with that observed in convalescent sera from C. trachomatis positive patients with non-gonococcal urethritis. Sera from healthy seronegative subjects were negative by blotting.