RNA Branching and Debranching in the Yeast Retrovirus-like Element Ty1

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Abstract
Ty elements of Saccharomyces cerevisiae are long terminal repeat (LTR) retroelements related to retroviruses. Normal levels of Ty1 transposition require Dbr1p, a cellular enzyme that cleaves 2′–5′ RNA bonds. We show that Ty1 RNAs lacking identifiable 5′ ends accumulate in virus-like particles (VLPs) in dbr1 mutants. Debranching this RNA in vitro with Dbr1p creates an uncapped version of the normal Ty1 RNA 5′ end. We show that the 5′ nucleotide (nt) of Ty1 RNA forms a 2′–5′ bond witha nt near the 3′ end of the same RNA, creating a lariat. The properties of the lariat suggest it forms by a novel mechanism and that branching and debranching may play roles in Ty1 reverse transcription at the minus-strand transfer step.