The effects of glutathione and adenosine on plasma membrane ATPases of the corneal endothelium. An hypothesis on the stimulatory mechanism of perfused glutathione upon deturgescence

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Abstract
Reduced glutathione (0.3 mM) stimulates the activity of Na-K activated ATPase (Na+K+ATPase) by 54% in plasma membranes prepared from bovine corneal endothelial cells. Oxidized glutathione has no effect on Na+K+ATPase activity in the same tissue, although it inhibits Mg activated ATPase (Mg2+ ATPase) by .apprx. 30%. Adenosine neither stimulates nor inhibits either Na+K+ATPase or Mg2+ATPase in these plasma membranes. The stimulatory effect of glutathione on deturgescence may stem from the direct reaction of the reduced form of the tripeptide on SH groups located on plasma membranes of corneal endothelial cells. Apparently these SH groups are part of the Na+K+ATPase complex.
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