Ex vivoAssessment of Lymphocyte Antioxidant Status Using the Comet Assay
- 1 January 1997
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research
- Vol. 27 (5) , 533-537
- https://doi.org/10.3109/10715769709065793
Abstract
Lymphocytes were isolated from volunteers before and after receiving a single supplement of vitamin C, vitamin E or β-carotene. The lymphocytes were treated with H2O2, and DNA strand breaks were measured by single cell gel electrophoresis (the comet assay). Significant protection against oxidative DNA damage was evident 2–4 h after vitamin C intake, and 18–24 h after consumption of the other antioxidants. Lymphocytes from smokers were more sensitive to DNA damage than those from non-smokers, and they showed at least as great a protective effect with antioxidants.Keywords
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