Failure to reverse cholera toxin induced intestinal secretion by agents which decrease mucosal cAMP
- 1 September 1979
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Physiology and Pharmacology
- Vol. 57 (9) , 1004-1010
- https://doi.org/10.1139/y79-150
Abstract
The feasibility of reducing intestinal secretion by the use of agents which decrease intestinal mucosal cAMP concentration has been investigated in the weanling pig and the rabbit. Three different agents for decreasing mucosal cAMP concentration were studied. The cyclic nucleotide phosphodiesterase activator, imidazole, significantly reduced mucosal cAMP concentrations only in the weanling pig. Intraluminal 2′-deoxyadenosine-3′-AMP inhibited adenylate cyclase and caused a decrease in mucosal cAMP concentration in both the pig and the rabbit. The introduction of the heat-stable enterotoxin of Escherichia coli into pig jejunal segments also gave lowered mucosal cAMP concentrations. While these three agents effectively reduced cAMP concentrations in intestinal mucosa, they were ineffective in reducing the net fluid secretory effects of cholera toxin. Secretion caused by cholera toxin apparently persists independent of the temporary changes in cAMP concentration which can be induced by pharmacological agents.This publication has 2 references indexed in Scilit:
- Cyclid 3':5'-nucleotide phosphodiesterase. Interconvertible multiple forms and their effects on enzyme activity and kinetics.Journal of Biological Chemistry, 1976
- ADENOSINE 3',5'-PHOSPHATE IN BIOLOGICAL MATERIALS .1. PURIFICATION AND PROPERTIES OF CYCLIC 3',5'-NUCLEOTIDE PHOSPHODIESTERASE AND USE SF THIS ENZYME TO CHARACTERIZE ADENOSINE 3',5'-PHOSPHATE IN HUMAN URINE1962