Determination of residues of enrofloxacin and its metabolite ciprofloxacin in chicken muscle by capillary electrophoresis using laser-induced fluorescence detection

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Abstract
A method for the residue analysis of the veterinary antimicrobial agent enrofloxacin and its active desethyl metabolite ciprofloxacin in chicken muscle tissue has been developed and validated. The detection of the analytes was performed by laser‐induced fluorescence (LIF) detection using a HeCd laser (λex = 325 nm) providing an enhancement in sensitivity and selectivity compared to conventional UV detection. The assay has been validated with satisfying results. The limits of quantification for enrofloxacin and ciprofloxacin were 5 νg/kg and 20 νg/kg, respectively, with a fivefold preconcentration yielded by a sample clean‐up with a simple liquid‐liquid extraction procedure. Calibration graphs were linear from 5 to 1000 νg/kg for enrofloxacin and from 20 to 1000 νg/kg for ciprofloxacin. The assay allows the detection of contaminated muscle samples at the required maximum residue limit of the European Union, which is 100 νg/kg for the sum of enrofloxacin and ciprofloxacin.

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