Biosynthesis of a γ3‐Melanotropin‐like Peptide in the Pars Intermedia of the Amphibian Pituitary Gland
- 1 August 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 126 (1) , 23-28
- https://doi.org/10.1111/j.1432-1033.1982.tb06740.x
Abstract
The biosynthesis of a .gamma.3-melanotropin-like peptide in the pars intermedia of the pituitary gland of the aquatic toad Xenopus laevis is revealed. Pulse-chase experiments in vitro showed that this product is synthesized through processing of a prohormone, pro-opiomelanocortin, and that it is released into the incubation medium. The peptide immunoprecipitated with antiserum to .gamma.3-melanotropin, appeared to be a glycopeptide and displayed melanotropic activity. This last observation together with the results of tryptic and chymotryptic peptide mapping of the newly synthesized product indicates that Xenopus .gamma.3-melanotropin is structurally different from the proposed mammalian .gamma.-melanotropins.This publication has 21 references indexed in Scilit:
- Biosynthesis of Two Structurally Different Pro‐opiomelanocortins in the Pars Intermedia of the Amphibian Pituitary GlandEuropean Journal of Biochemistry, 1982
- Biosynthesis of Pairs of Peptides Related to Melanotropin, Corticotropin and Endorphin in the Pars Intermedia of the Amphibian Pituitary GlandEuropean Journal of Biochemistry, 1982
- Isolation and characterization of a γ1‐melanotropin‐like peptide from bovine neurointermediate pituitaryFEBS Letters, 1981
- The isolation and characterization of γ3-melanotropin from the neurointermediary lobe of the rat pituitaryBiochemical and Biophysical Research Communications, 1981
- Structure and Chemistry of the Peptide Hormones of the Intermediate LobePublished by Wiley ,1981
- A radioimmunoassay for γ1-melanotropin and evidence that the smallest pituitary γ-melanotropin is amidated at the COOH-terminusBiochemical and Biophysical Research Communications, 1980
- Pituitary immunoreactive γ-melanotropins are glycosylated oligopeptidesNature, 1980
- Synthesis and biological activity of four γ-melanotropin derived from the cryptic region of the adrenocorticotropin/β-lipotropin precursorLife Sciences, 1979
- RE-INTRODUCTION AND EVALUATION OF AN ACCURATE, HIGH CAPACITY BIOASSAY FOR MELANOCYTE-STIMULATING HORMONE USING THE SKIN OF ANOLIS CAROLINENSIS IN VITROJournal of Endocrinology, 1975
- Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomesBiochemical and Biophysical Research Communications, 1975