The effects of the ring fragmentation product of thymidine C5-hydrate on phosphodiestrases and klenow (exo-)fragment

1 January 1995

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 23 (22) , 4642-4648
https://doi.org/10.1093/nar/23.22.4642

Abstract

N-(2-Deoxy-β-D-erythro-pentofuranosyl)-N-3-(2Rhydroxyisobutyric acid)urea (α-R-hydroxyisobutyric acid)urea (αR-hydroxy-β-ureldoisobutyric acid, B) was site specifically incorporated into a series of oligonucleotides via the ammonolysis of biopolymers containing 5R-thymidlne C5-hydrate (3). α-R-hydroxy-β-ureidoisobutyric acid (8) Inhibits snake venom phosphodiesterase, Xλexonuclease and Klenow (exo^∼) fragment. Kinetic measurements for insertion of nucleotldes opposite 8 by Klenow (exo^∼) fragment Indicate that this lesion is Instructive.

Keywords

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