Multielement trace-element speciation in metal-biomolecules by chromatography coupled with ICP–MS

27 April 2002

journal article
Published by Springer Nature in Analytical and Bioanalytical Chemistry

Vol. 373 (6) , 412-421
https://doi.org/10.1007/s00216-002-1278-y

Abstract

The state-of-the-art of multi-element speciation in biological materials by hybrid techniques with inductively coupled plasma–mass spectrometric (ICP–MS) detection is critically reviewed with special attention to multi-metal speciation in metallothioneins (MT) and metallothionein-like proteins (MLP). The most common separation techniques used for the purpose include conventional size-exclusion chromatography (SEC) (with off-line detection) and anion-exchange (AE) or reversed-phase (RP) HPLC coupled on-line to ICP–MS. Advantages and limitations of the different commercial element-selective ICP–MS detectors, including the quadrupole (Q), double focusing (DF), and time-of-flight (TOF) mass analysers, are discussed. Procedures reported for speciation analysis, by use of hybrid techniques, for multi-metal speciation in mussel MLP and rabbit liver MT are illustrated with special emphasis on work performed in the authors’ laboratory.

Keywords

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