Noncovalent Labeling of Biomolecules with Red and Near- Infrared Dyes
Open Access
- 28 February 2004
- Vol. 9 (3) , 40-49
- https://doi.org/10.3390/90300040
Abstract
Biopolymers such as proteins and nucleic acids can be labeled with a fluorescent marker to allow for their detection. Covalent labeling is achieved by the reaction of an appropriately functionalized dye marker with a reactive group on a biomolecule. The recent trend, however, is the use of noncovalent labeling that results from strong hydrophobic and/or ionic interactions between the marker and biomolecule of interest. The main advantage of noncovalent labeling is that it affects the functional activity of the biomolecule to a lesser extent. The applications of luminescent cyanine and squarylium dyes are reviewed.Keywords
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