Radiation induced formation of giant cells inSaccharomyces uvarum III: Effect of X-rays on nuclear division

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Abstract
Spindle formation and nuclear division of budding and irradiated yeast cells (Saccharomyces uvarum) was investigated by fluorescence microscopy of protoplasted cells. Protoplasts were treated with antitubulin antibodies and DAPI, a fluorescent dye staining DNA. In budding yeast cells, duplication of spindle pole bodies as well as formation of complete 1-µm spindles and elongated 8-µm spindles were documented. In X-irradiated cells, spindle pole bodies were duplicated as well, forming the complete 1-µm spindle. Nuclei of giant cells have lost the elongation ability and remain in a “normal” G2-phase state, thus preventing nuclear as well as cellular division.