Acquired Resistance in Hypersensitive Tobacco against Tobacco Mosaic Virus, Induced by Plant Cell Wall Components

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Abstract
Isolated cell walls from Nicotiana tabacum L. cv. Xanthi‐nc were partially digested with enzymes present in the fungal preparation cellulase Onozuka R‐10. Released carbohydrate material was separated from the enzymes by gel filtration and fractions were injected into the intercellular spaces of one half of each of several Xanthi‐nc leaves. Two to three days after injection the whole leaves were inoculated with TMV. A large reduction in mean lesion diameter was observed in the injected areas compared with those in untreated tissue.