INCREASED CALCIUM SEQUESTRATION BY SARCOPLASMIC RETICULUM IN SMALL MUSCULAR ARTERIES IN YOUNG SPONTANEOUSLY HYPERTENSIVE RATS

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Abstract
Summary: 1. Sarcoplasmic reticulum (SR) in small resistance arteries plays a role in the regulation of the cytosolic free calcium concentration by sequestration of calcium from cytoplasm.2. To examine the contribution of calcium (Ca2+) sequestration by the SHR to both contraction and relaxation in young spontaneously hypertensive rats (SHR), we measured evoked tension before and after depletion of SR Ca2+ stores in the rings of the first branch of superior mesenteric artery in 5 week old SHR and age‐matched Wistar‐Kyoto (WKY) rats. Contractile responses were induced by 40 mmol/L potassium and 10 mmol/L caffeine before and after the treatment with ryanodine or thapsigargin, which depletes SR calcium stores.3. The magnitude of potassium‐induced contraction was almost the same in both strains.4. Ryanodine and thapsigargin did not affect the resting tension and almost decreased caffeine‐induced contraction in both strains.5. After the treatment with ryanodine or thapsigargin, the magnitude and the rate of potassium‐induced contraction were augmented greatly in SHR, but not in WKY.6. The relative relaxation rate after exposure of potassium was significantly slowed in SHR by ryanodine or thapsigargin, but only slightly in WKY.7. These results suggest that Ca2+ sequestration by the SR in SHR was greater than in WKY. Therefore, it is concluded that SR plays an important role in preventing the development of hypertension in SHR via a buffering effect on the elevation of cytosolic free Ca2+.