THE ONSET OF POLYMORPHONUCLEAR LEUKOCYTE MEMBRANE-STIMULATED METABOLIC-ACTIVITY

  • 1 January 1981
    • journal article
    • research article
    • Vol. 43  (4) , 733-739
Abstract
The time course of human polymorphonuclear leukocyte oxidative metabolism following membrane stimulation by 4 different agents was examined using the techniques of luminol- and lucigenin-dependent chemiluminescence. After addition of opsonized zymosan, phorbol myristate acetate or digitonin to polymorphonuclear leukocytes there was a lag period of 35-55 s before the onset of chemiluminescence. After addition of the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP), the lag period before chemiluminescence was < 10 s. Luminol-dependent chemiluminescence was reduced by superoxide dismutase and almost abolished by sodium azide. The inhibitory effect of the latter was less marked when using FMLP. Lucigenin-dependent chemiluminescence was inhibited by superoxide dismutase and enhanced by sodium azide. Cytochalasin B reduced zymosan and digitonin stimulated chemiluminescence but increased FMLP stimulated chemiluminescence. The results are discussed in relation to previous reports of the onset of polymorphonuclear leukocyte metabolic activity using other techniques.

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