A13C NMR study of the application of [U‐13C] succinate for metabolic investigations in rabbit renal proximal convoluted tubular cells

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Abstract
The fate of the 13C label arising from [ U‐13C ] succinate in rabbit renal proximal convoluted tubular (PCT) cells, incubated with [U‐13C]succinate, was investigated by 13C NMR spectroscopy of the perchloric acid extracts of the cell pellets. The 13C label of [U‐13C] succinate was predominantly metabolized into glutamate, glutamine, and into glucose, lactate, and alanine, which is the consequence of a carbon efflux of the label from the Krebs cycle at the stage of α‐ketoglutarate and oxaloacetate, respectively. © 1990 Academic Press, Inc.