Retention Mechanism in Ligand exchange Chromatography of α-Amino Acids: Improved Resolution of Racemates with A New Mutildentate Polymeric Packing

Abstract
On the basis of the expected mechanism of chiral recognition in ligand exchange chromatography, a new chiral packing was prepared and tested. The optically active phase is a copolymer of acrylamide and of a vinylpyridine monomer substituted by anL-pro-line moiety. The packing is obtained by simple coating of a chro-matographic silica followed by a convenient complexation with cupric ions. The selectivity factor, α, for such a packing is high : it is between 2 and 8 for most amino acids.

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