The Effects of Pre- and Postirradiation Centrifugation on the Chromosomes of Tradescantia and Vicia

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Abstract
It has been observed that in Tradescantia microspores and in Vicia seed preirradiation centrifugation decreases the number of aberrations as compared to noncentrifuged controls. Postirradiation centrifugation increases aberrations, provided that the centrifuging occurs before the chromosome breaks rejoin. These phenomena have been explained on the basis of physically induced compression or movement of the chromosomes. Cells of Tradescantia irradiated in N show no increase of aberration yield caused by centrifugation. This is explained on the basis that rejoining occurs in a shorter time after anaerobic irradiation, as is the case in Vicia.