Transformation of Azotobacter vinelandii with Plasmids RP4 (IncP-1 Group) and RSF1010 (IncQ Group)
- 1 June 1981
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 146 (3) , 1154-1157
- https://doi.org/10.1128/jb.146.3.1154-1157.1981
Abstract
Multicopy plasmid RSF1010 and four of its in vitro-constructed derivatives were mobilized by the self-transmissible RP4 plasmid into Azotobacter vinelandii UW. Modifications of the Escherichia coli transformation procedure of Cohen et al. (Proc. Natl. Acad. Sci. U.S.A. 69: 2110–2114, 1972) allowed transformation of A. vinelandii strains UW and ATCC 12837 with purified RP4 or RSF1010 deoxyribonucleic acid.This publication has 19 references indexed in Scilit:
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