Infection of Protoplasts from Chenopodium Quinoa with Cowpea Mosaic and Cymbidium Ringspot Viruses

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Abstract
Mesophyll protoplasts were prepared from Chenopodium quinoa plants and infected with cowpea mosaic virus (CPMV) or cymbidium ringspot virus (CyRSV) using inocula containing either polyethylene glycol or poly-l-ornithine. The level of infection was estimated by fluorescent antibody staining and by spot hybridization assay. About 25% of the protoplasts became infected with CPMV and about 35% with CyRSV. Yields of progeny RNA were about 700 ng of CPMV RNA and 3 µg of CyRSV RNA per 106 protoplasts. Cytopathic structures found in C. quinoa protoplasts inoculated with CPMV or CyRSV were similar to the structures found in CPMV-infected cowpea protoplasts or in CyRSV-infected C. quinoa plants respectively.

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