The regulation of sclerotium initiation in Sclerotium rolfsii by glucose and cyclic AMP

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Abstract
S. rolfsii, grown in a shaken submerged culture, developed a potential for the formation of sclerotia. This potential was inhibited by the presence of glucose in the culture medium. The cultures were capable of producing sclerotia, after transfer to stationary incubation, when glucose was exhausted and the biomass reached its maximal level. The level of cAMP sharply increased upon glucose depletion and decreased upon glucose addition. S. rolfsii was grown in a chemostat with glucose of limiting concentration at a dilution rate of 0.05/h. Samples from the chemostat possessed the potential to produce initials when poured into empty Petri plates. Addition of glucose to these plates changed the fungal growth pattern from further differentiation to vegetative growth. Glucose supplementation to clearly differentiated initials did not inhibit further development. During morphogenetic events, endogenous substrates, e.g., cell wall components, were utilized.
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