The isolation and partial characterization of the serum lipoproteins and apolipoproteins of the rainbow trout

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Abstract
VLD (very-low-density), LD (low-density) and HD (high-density) lipoproteins were isolated from the serum of trout (Salmo gairdneri Richardson). Each lipoprotein class resembled that of the human in immunological reactivity, electrophoretic behavior and EM appearance. Trout LD lipoprotein, however, was of greater density than human LD lipoprotein. The trout lipoproteins have lipid compositions which are similar to those of the corresponding human components, except for their high contents of long-chain unsaturated fatty acids. HD and LD lipoproteins were immunologically non-identical, whereas LD lipoproteins possessed antigenic determinants in common with VLD lipoproteins. VLD and HD lipoproteins each contained at least 7 different apoproteins, whereas LD lipoprotein was composed largely of a single apoprotein which resembled human apolipoprotein B. At least 1, and possibly 3, apoproteins were common to both VLD and HD lipoproteins. The major apoprotein of trout HD lipoprotein showed features which resemble human apoprotein A-1. The broad similarity between the trout and human lipoprotein systems suggests that both arose from common ancestral genes early in evolutionary history.