Horse brain acylphosphatase: Purification and characterization
- 15 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 236 (1) , 209-216
- https://doi.org/10.1016/0014-5793(88)80316-8
Abstract
Two structurally different acylphosphatases found in horse brain were purified; they were not immunologically related. The molecular masses were almost identical and the kinetic parameters were rather similar. The data reported indicate that one of the purified brain acylphosphatases and an enzyme, previously isolated from horse muscle, are the same protein. The presence of this acylphosphatase form in the brain has not been reported before. The other acylphosphatase seemed to be the same as the enzyme which had been purified from calf brain and partially characterized by Diederich and Grisolia [(1969) J. Biol. Chem. 244, 2412–2417]. Furthermore, this enzyme seems to be identical to the acylphosphatase recently purified in our laboratory from human erythrocytes.Keywords
This publication has 10 references indexed in Scilit:
- Acylphosphatase levels of human erythrocytes during cell ageingMechanisms of Ageing and Development, 1987
- A new acylphosphatase isoenzyme from human erythrocytes: purification, characterization, and primary structureBiochemistry, 1986
- Affinity chromatographic purification of horse muscle acylphosphatase: Evidence of the existence of multiple molecular formsArchives of Biochemistry and Biophysics, 1983
- The complete amino acid sequence of horse muscle acylphosphatase.Journal of Biological Chemistry, 1980
- A new synthesis of benzoyl phosphate: A substrate for acyl phosphatase assayCellular and Molecular Life Sciences, 1976
- Properties of Pure Acyl Phosphatase from Bovine BrainJournal of Biological Chemistry, 1969
- Horse muscle acyl phosphatase: Purification and some propertiesArchives of Biochemistry and Biophysics, 1969
- High resolution acrylamide gel electrophoresis of histonesArchives of Biochemistry and Biophysics, 1969
- Spectroscopic Determination of Tryptophan and Tyrosine in Proteins*Biochemistry, 1967
- Continuous optical assay of acylphosphatase with benzoylphosphate as substrateCellular and Molecular Life Sciences, 1966