Pneumonia Virus of Mice (PVM) in Cell Culture.

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Abstract
Evidence is presented for the multiplication of pneumonia virus of mice (PMV) in suckling hamster kidney cell culture monolayers. The cell culture-derived virus resembled the original lung-derived virus in all properties examined including pathology, serological reactivity, trypsin sensitivity and combination with a hemagglutination inhibitor. In cell culture virus was produced in low titer and caused a cytopathic effect. Infected cultures hemadsorbed mouse erythrocytes. Tissue components which combine with the virus in vivo were not detected in cell culture fluids. Propagation in cell culture appears to free PVM from the Riley Agent present as a contaminant of the mouse passaged virus.