Immobilization of Enzymes on Methacrylamide-Based Copolymers
- 1 February 1997
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry & Biotechnology
- Vol. 27 (1) , 39-46
- https://doi.org/10.1080/10826069708001276
Abstract
A series of reactive polymeric supports for the covalent binding of enzymes was prepared from methacrylamide in conjunction with various copolymerized monomers. In all cases the specific surface area of the prepared copolymer particles increased with the ratio of crosslinker to monomer. The polymers containing oxirane and aldehyde groups were promising for direct binding of β-galactosidase, whereas the supports containing ammo groups also qualified for enzyme immobilization with glutaraldehyde.Keywords
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