Somatic antigens of Pseudomonas aeruginosa. The structure of the O-specific polysaccharide chains of P. aeruginosa 011 (Lanyi) lipopolysaccharides

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Abstract
Lipopolysaccharides were isolated from the phenol layer on aqueous phenol extraction of cells of P. aeruginosa 011 (Lanyi classification), strains 170021 and 170040. On mild acid degradation of the lipopolysaccharides, with the subsequent gel-filtration on Sephadex G-50, neutral O-specific polysaccharides made up of 6-deoxysugars alone were obtained. Two 2-acetamido-2,6-dideoxy-L-galactose (LFucNAc), 2-acetamido-2,6-dideoxy-D-glucose (DQuiNAc) and L-rhamnose (LRha) residues were found to be the components of the strain 170021 polysaccharide repeating units; those of strain 170040 contained the same monosaccharides, but, instead of 2-acetamido-2,6-dideoxy-D-glucose residue, that of 2-acetamido-2,6-dideoxy-D-galactose (DFucNAc) was present. On the basis of the 13C NMR data, methylation analysis and 3 successive Smith degradations the following structures were determined for the polysaccharide repeating units: strain 170021, .fwdarw. 2) LRha(.alpha.1 .fwdarw. 3)LFucNAc(.alpha.1 .fwdarw. 3)LFucNAc(.alpha.1 .fwdarw. 3)D-QuiNAc(.beta.1 .fwdarw.; strain 170040, .fwdarw. 2)LRha(.alpha.1 .fwdarw. 3)LFucNAc-(.alpha.1 .fwdarw. 3)LFucNAc(.alpha.1 .fwdarw. 3)DRucNAc(.beta.1 .fwdarw.; differing from one another by configuration of C-4 of 2-acetamido-2,6-dideoxy-D-hexopyranose only.