Hemoglobin Miyashiro (β 23[B5] Val → Gly) an Electrophoretically Silent Variant Discovered by the Isophopanol Test

Abstract
A new unstable hanoglobin was discovered in a Japanese boy without any signs of hemolytic disease during screening of patients by the isopropanol test. The abnormal hemoglobin was found in three generations. This slightly unstable hemoglobin could not be separated from Hb A by electrophoresis. All carriers showed an increased whole blood oxygen affinity. Structural studies demonstrated a previously unreported amino acid substitution, β23[B5] valine → glycine. This new unstable variant has been named Hb Miyashiro.