Evidence for sialidase hydrolyzing gangliosides GM2 and GM1 in rat liver plasma membrane
- 6 October 1986
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 206 (2) , 223-228
- https://doi.org/10.1016/0014-5793(86)80985-1
Abstract
Rat liver plasma membrane removed sialic acid from mixed bovine brain gangliosides more efficiently than from sialyllactose and orosomucoid with an optimal pH of 4.5. When individual gangliosides, each labeled with [14C]sialic acid or [3H]sphingosine, were tested, not only GDla and GM3 but also GM2 and GM1, both of which had been considered to resist mammalian sialidases, were desialylated. The products of GM2 and GM1 hydrolysis were identified as asialo-GM2 and asialo-GM1, respectively, by thin-layer chromatography.Keywords
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