Comparison of Characteristics of the nodX Genes from Various Rhizobium leguminosarum Strains
Open Access
- 1 March 1999
- journal article
- research article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 12 (3) , 252-258
- https://doi.org/10.1094/mpmi.1999.12.3.252
Abstract
We have analyzed the nucleotide sequences of the nodX genes from two strains of Rhizobium leguminosarum bv. viciae able to nodulate Afghan peas (strains A1 and Himalaya) and from two strains of R. leguminosarum bv. trifolii (ANU843 and CSF). The nodX genes of strains A1 and ANU843 were shown to be functional for the induction of nodules on Afghan peas. To analyze the cause of phenotypic differences of strain A1 and strain TOM we have studied the composition of the lipochitin-oligosaccharides (LCOs) produced by strain A1 after induction by the flavonoid naringenin or various pea root exudates. The structural analysis of the LCOs by mass spectrometry revealed that strain A1 synthesizes a family of at least 23 different LCOs. The use of exudates instead of naringenin resulted only in quantitative differences in the ratios of various LCOs produced.Keywords
This publication has 27 references indexed in Scilit:
- Restriction of Host Range by the sym2 Allele of Afghan Pea Is Nonspecific for the Type of Modification at the Reducing Terminus of Nodulation SignalsMolecular Plant-Microbe Interactions®, 1998
- Mutation or Increased Copy Number of nodE Has No Effect on the Spectrum of Chitolipooligosaccharide Nod Factors Made By Rhizobiumleguminosarum bv. trifoliiPublished by Elsevier ,1995
- Host Specificity ofRhizobium leguminosarumis Determined by the Hydrophobicity of Highly Unsaturated Fatty Acyl Moieties of the Nodulation FactorsMolecular Plant-Microbe Interactions®, 1995
- Structural identification of metabolites produced by the NodB and NodC proteins of Rhizobium leguminosarumMolecular Microbiology, 1994
- Resistance to nodulation of cv. Afghanistan peas is overcome by nodX, which mediates an O‐acetylation of the Rhizobium leguminosarum lipo‐oligosaccharide nodulation factorMolecular Microbiology, 1993
- Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.Proceedings of the National Academy of Sciences, 1980
- Strain Identification in Rhizobium Using Intrinsic Antibiotic ResistanceJournal of Applied Bacteriology, 1979
- High frequency transfer of nodulating ability between strains and species of RhizobiumNature, 1978
- Symbiotic specialisation in pea plants: The requirement of specific Rhizobium strains for peas from AfghanistanAnnals of Applied Biology, 1978
- The Wall of Rhizobium leguminosarum in Bacteroid and Free-living FormsJournal of General Microbiology, 1977