Effect of Estradiol-17β and Luteinizing Hormone on Progesterone Secretion by Luteal Cells from Early Pregnant, Estradiol Benzoate-Treated and Human Chorionic Gonadotropin-Treated Sows

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Abstract
The present study was conducted to examine the effect of estradiol 17β (E2) and luteinizing hormone (LH) on progesterone (P4) secretion by luteal cells from early pregnant, estradiol benzoate (EB)-treated and hCG-treated sows and to compare the sensitivity of these cells to used exogenous hormones in vitro. Trypsin-dispersed luteal cells (5 X104 cells/ml) were incubated with E2 (doses: 0.001, 0.01, 0.1, 1 and 5 μg/ml), with LH (doses: 0.01, 0.1 and 1 μg/ml) and with E2 (1 μg) plus LH (0.01, 0.1 and 1 μg doses). Control cultures were incubated without exogenous hormones. The P4 level was estimated by radioimmunoassay after 1, 3 and 6 hour incubation. Luteal cells from hCG-treated sows released significantly more (P < 0.05) P4 than the cells from EB-treated and pregnant pigs, the luteal cells frompregnant sows produced the least P4 amounts (P < 0.05). The cells from pregnant pigs enhanced P4 secretion under influence of higher doses of LH and the cells from EB-treated sows under lower LH doses. The cells from hCG-treated animals did not respond to LH. E2 did not change P4 production by luteal cells from sows in the three investigated physiological stages, only the 0.01 μg E2 dose stimulated (P < 0.05) P4 release by the cells from pregnant pigs. E2 inhibited luteotropic LH action upon P4 secretion by luteal cells from pregnant and EB-treated sows. The results suggest that the luteal cells from early pregnant, EB-treated and hCG-treated sows differ from each other in steroidogenic potency and in the strength of reaction to E2 and LH.

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