Cytochemical Studies on the Effect of Antigen on Peritoneal Exudate Cells from Guinea Pigs with Delayed Hypersensitivity

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Abstract
The intraperitoneal injection of anti-gen into guinea pigs with delayed sensitivity produced cytochemical evidence of decreased activity of the enzymes lactate dehydrogenase, glutamate dehydrogenase, glucose-6-phosphate dehydrogenase and 6-phosphogluconata dehydrogenase in peritoneal macrophages. However, conventional biochemical analysis revealed no decrease in lactate dehydrogenase. There was similar cytochemical evidence of decreased DPNH and TPNH diaphorase activity. These results in-dicated that the fundamental lesion was a decreased cell permea-bility and this was reversed by increasing cell permeability by in-sulin, histamine and K+ ions. The changes were immunologically specific and the association with delayed hypersensitivity rather. than classical humoral antibody is discussed.

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