Purification, antitumor activity, and structural characterization of .BETA.-1,3-glucan from Peziza vesiculosa.

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Abstract
An antitumor .beta.-1,3-glucan (PVG) was purified from PVA-S by sequential use of .alpha.-amylase digestion, ethanol precipitation, ion exchange chromatography on diethyl aminoethyl Sephadex A-25, ammonium sulfate fractionation, and gel filtration on Sepharose CL-2B. PVG showed [.alpha.]D +28.4 (water). From the results of methylation analysis and carbon 13 nuclear magnetic resonance spectroscopy, PVG is a .beta.-1,3-glucan branched at position 6 of every fifth 3-substituted .beta.-glucosyl unit. PVG formed a complex with Congo Red in neutral and dilute alkali solution, but this complex was dissociated at more than 0.2 N NaOH. PVG showed potent antitumor activity against the solid form of sarcoma 180 tumor in ICR mice.