Fracture-Flip: New High-Resolution Images Of Cell Surfaces After Carbon Stabilization Of Freeze-Fractured Membranes
Open Access
- 1 August 1988
- journal article
- research article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 90 (4) , 531-541
- https://doi.org/10.1242/jcs.90.4.531
Abstract
We introduce fracture-flip, a method of obtaining high-resolution views of membrane surfaces. The method, a corollary of label-fracture, is based on the stabilization of the exoplasmic halves of membranes by carbon evaporation. Inversion of these casts followed by Pt/C imaging leads to new views of the cell surface at macromolecular resolution.This publication has 14 references indexed in Scilit:
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