The effects of saturated and polyunsaturated dietary lipids on intestinal mucosal cell proliferation were investigated in rats. Animals were randomly allocated to three groups of 10, and fed fibre-free diets containing lipid in the form of lard, corn oil or fish oil at a level of 80 g/kg. Total energy intake was kept constant by pair-feeding. After 14 days the crypt cell production rates (CCPR) at two sites in the small intestine, one site in the caecum and two sites in the colon were determined by the metaphase arrest technique, together with circulating levels of enteroglucagon and gastrin and parameters of mucosal morphology. Consumption of fish oil led to lower CCPR compared to corn oil at all sampling positions except the caecum (Treatment∼ 50% of control values; P < 0.05). In animals fed lard the CCPR in the small bowel was not significantly different to that of animals fed corn oil but their rates were lower in the colon. Post-prandial enteroglucagon and gastrin levels were lower in animals fed fish oil compared to the other two groups. These results suggest that polyunsaturated fatty acids of the n – 3 series may support a relatively Low rate of crypt cell proliferation in some regions of the alimentary tract and might thereby tend to suppress the progression of colorectal neoplasms.