IDENTIFICATION OF CHROMOSOMES IN INTRASPECIFIC HYBRID CELLS BY G-BANDING METHOD

  • 1 January 1975
    • journal article
    • research article
    • Vol. 21  (3) , 196-+
Abstract
The karyotype of HY 5/3-4 hybrid cells formed by fusion of diploid and heteroploid murine cells (LS/BL (lymphosarcoma strain) .times. R-AG/20 (fibroblast L cells resistant to 8-azaguanine)) consisted of 60-75 chromosomes (modal number 72). The G-banding technique made it possible to identify the origin of the chromosomes. Due to chromosomal segregation the hybrid genome probably lost preferentially the chromosomes derived from the diploid parental line LS/BL. Even after long-term culture in vitro, the chromosomal complement of hybrid cells retained all bi-armed chromosomes and most of the telocentrics, derived from the heteroploid parental cells.

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