Induction of erythroid differentiation in murine erythroleukemia cells by N-substituted polymethylene diamides

Publisher Website
Google Scholar
Abstract
Various N‐substituted polymethylene diamides were synthesized and tested for their potency to induce erythroid differentiation in murine erythro‐leukemia cells. N,N,N′,N′‐tetramethyl‐1.6‐hexane‐dicarboxamide (IIc) was the most potent inducer among 15 compounds tested. The effectiveness of this compound was similar to that of hexamethylene bisacetamide(HMBA). HMBA has a different amide linkage order from that of IIc. HMBA and IIc at a concentration of 5 mM had similar effects on the cell growth rate and induced a similar frequency of benzidine‐positive cells. Hower, hemoglobin production was 1.5 times more effective with IIc. Polymethylene diester, diamide, dihydrazide and dianilide had no effect on the induction of hemoglobin synthesis. The N‐alkylated amide group appears to be required for induction of differentiation in murine erythroleukemia cells.