STUDIES ON BRAIN GANGLIOSIDES: I. THE ISOLATION AND COMPOSITION OF A TRISIALOGANGLIOSIDE

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Abstract
Brain gangliosides, extracted with tetrahydrofuran and then purified, contained 1.03 ± 0.05 μmoles N-acetylneuraminic acid (NANA) per mg. They separated into five major and four minor components on thin-layer chromatograms.Gangliosides extracted from acetone powders of brain with hot chloroform–methanol separated on cellulose columns into two crude fractions (I and II). Purified, fraction II was a trisialoganglioside containing 1.29 μmoles NANA/mg. It was the slowest-moving component on thin-layer chromatograms. Fraction I was a mixture of mono- and di-sialogangliosides and contained the other chromatographic components. The molar ratio of hexose:ceramide of all preparations was close to 3:1. The molar ratio of galactose:glucose also was close to 3:1.The chromatography of gangliosides on cellulose columns varies significantly with different kinds of cellulose. Fraction I gangliosides appeared as one peak with Schleicher and Schuell pulp but ran as two separate peaks on Whatman cellulose. A modified method is described for the assay of sphingosine in gangliosides.

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