In vivomeasurements of the production rates of bacteria in the rumen

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Abstract
The production rates of bacteria have been measured in the rumen of zebu calves and buffalo calves. The animals were fed green oat continuously at 2 h intervals. [35S]sodium sulphate was fed to the animals for 5 days at 2 h intervals by mixing in the feed. On the sixth day the radioactive feed was stopped and replaced by non-radioactive feed. The decline in the specific radioactivity of bacterial cells in the rumen as a function of time was taken for calculatingt/2. Simultaneously rumen volume was determined and pool size of bacterial cells was calculated in the rumen. A mathematical equation was applied to calculate the production rates of bacteria. The production rates of bacteria, on average, were 100·5 and 72·3 mg/min for buffalo calves and zebu calves, respectively.

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