Characterisation of the expression and post‐translational processing of the preprotachykinin‐I gene and the regulated release of tachykinins by the RINm5F cell‐line

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Abstract
Normal transcription and postranslational processing of the preprotachykinin (PPT)‐I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell‐line, RINm5F, has been demonstrated, using radioimmunoassays (RlAs), reversed‐phase (rp)HPLC and Northern blot analysis. This is the first stable cell‐line found to express the PPT‐I gene and provides an opportunity for investigating PPT‐I gene expression and tachykinin biosynthesis. RIN5mF cells are a model for the pancreatic beta‐cell, which is not known to exhibit PPT‐I gene expression which may, therefore, be a feature of the transformed state of these cells. These data may imply that the tachykinins are important in pancreatic islet embryogenesis.