Modelling the PCR amplification process by a size-dependent branching process and estimation of the efficiency
- 1 June 2004
- journal article
- Published by Cambridge University Press (CUP) in Advances in Applied Probability
- Vol. 36 (2) , 602-615
- https://doi.org/10.1239/aap/1086957587
Abstract
We propose a stochastic modelling of the PCR amplification process by a size-dependent branching process starting as a supercritical Bienaymé-Galton-Watson transient phase and then having a saturation near-critical size-dependent phase. This model allows us to estimate the probability of replication of a DNA molecule at each cycle of a single PCR trajectory with a very good accuracy.Keywords
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