Macroaffinity Ligand‐Facilitated Three‐Phase Partitioning (MLFTPP) of α‐Amylases Using a Modified Alginate

1 January 2003

journal article
research article
Published by Wiley in Biotechnology Progress

Vol. 19 (2) , 493-494
https://doi.org/10.1021/bp025619e

Abstract

The crude extracts of α‐amylases when mixed with alginate, tert‐butyl alcohol, and ammonium sulfate resulted in an interfacial precipitate containing polymer‐bound amylase. The precipitate was dissolved in 1 M maltose to recover α‐amylase activity. The recovery of α‐amylases were 74%, 77%, and 92% in the case of Bacillus amyloliquefaciens, wheat germ, and porcine pancreas, respectively. All purified preparations showed a single band on SDS‐PAGE.

Keywords

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