Enrichment and Plating Methodology for Salmonella Detection in Food. A Review1

Abstract
Much research concerned with enrichment and plating methodology to detect salmonella contamination in foods has been reported by many scientists. This review brings reported findings of major proponents together into one text for greater understanding and appreciation of the complexity of the problem. Discussed in this review are reported applications and mechanisms of 11 enrichment media and eight plating media, incubation temperatures and times, and serotype specificity involving enrichment and plating media. Also, enrichment emulsifying agents, agitation during incubation, sample type, and level and proportion of salmonellae and competitors as related to salmonella enrichment are discussed. Other factors related to salmonella recovery, such as multiple media methods, preparation methods, storage of media, and media brand are included in this review, as well as a discussion of the methodology dilemma and some general recommendations for future direction.