Rapid enzyme-linked immunosorbent assay (ELISA) with a visual end-point for detecting quinine in urine, serum and dried blood spots
- 1 January 1987
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 112 (10) , 1437-1439
- https://doi.org/10.1039/an9871201437
Abstract
A rapid and simple assay has been developed for the detection of quinine in biological fluids (e.g., urine, serum and dried blood spots). The assay has a coloured end-point and a limit of sensitivity of 3 µg l–1, using 5-µl samples. No cross-reactivity is observed with examples of commonly administered drugs. The assay was used to screen samples of dried blood spots and urine from a volunteer after taking a dose of 300 mg of quinine. The assay requires no sophisticated laboratory equipment or instrumentation to give a qualitative assessment of the presence of quinine, but may be used with a simple portable microtitre plate reader to give quantitative measurements if required.Keywords
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