Use and Advantages of Ethanol Solution of Alizarin Red S Dye for Staining Bone in Fishes
- 1 January 2000
- journal article
- Published by American Society of Ichthyologists and Herpetologists (ASIH) in Ichthyology & Herpetology
- Vol. 2000 (1) , 300-301
- https://doi.org/10.1643/0045-8511(2000)2000[0300:uaaoes]2.0.co;2
Abstract
A solution of 75% ethyl alcohol (ETOH) and alizarin red S can be substituted for KOH-alizarin red S to stain bone. ETOH-alizarin, unlike KOH-alizarin, does not damage muscle or cartilage. ETOH-alizarin and acidified alcian blue (a cartilage stain) can be used in any order and as often as required to stain bone and cartilage in a single preparation that has not been exposed to KOH-alizarin or trypsin, remembering that the acidic effects of the alcian blue solution should be neutralized to prevent decalcification of weakly ossified elements.Keywords
This publication has 1 reference indexed in Scilit:
- Clearing and dyeing fish for bone studyZoologica : scientific contributions of the New York Zoological Society, 1934