A NEW BACTERIUM (HEMOPHILUS PISCIUM N. SP.) FROM ULCER DISEASE OF TROUT

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Abstract
H. piscium was isolated repeatedly at Kearneysville, W. Va., from brook trout suffering from ulcer disease. It can be isolated and cultivated on media containing fresh blood or extract or peptone prepd. from fish tissues and sterilized by filtration. It ferments some mono- and disaccharides rapidly with the production of acid and does not liquefy gelatin or change litmus milk. Under the exptl. conditions, the presence of the X and V factors, singly or in combination, was not sufficient for the growth of this organism. It appeared to require some thermolabile substance assumed to be present in potato extract, fish peptone, and rabbit erythrocytes. Rabbit serum did not support growth. Growth was enhanced by the addition of starch to the basal medium containing potato extract or fish peptone. This organism is pathogenic to brook, brown, and rainbow trout, but not to bluegill sunfish or frog larvae.
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