Failure to Demonstrate Fibrotic Changes in the Skin of Mice Injected with Glycosaminoglycan Fractions from the Urine of Scleroderma Patients

Abstract
Glycosaminoglycan (GAG) fractions isolated from the urine of scleroderma patients were compared with GAGs from control subjects for their ability to induce fibrotic changes in the skin of mice after intraperitoneal injection. Contrary to previous reports, however, no fibrotic changes were detectable, either visibly or in terms of skin tissue thickness. The ability to obtain consistently authentic biologically active factors from this source is therefore questioned.

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