Intraluminal suture occlusion of the middle cerebral artery in Spontaneously Hypertensive rats

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Abstract
In models of middle cerebral artery occlusion using intraluminal suture, the size and the distribution of ischemic injury vary considerably among laboratories. In transcranial models of cerebral ischemia, a more consistent cerebral ischemic lesion is seen in Spontaneously Hypertensive rats (5HR). In the present study, we performed intraluminal suture occlusion ofthe MCA in SHR and compared its reproducibility with those in Sprague-Dawley (SD) rats. Male 5HR and SO rats were anesthetized with halothane and subjected to 2 h of temporary middle cerebral artery occlusion by an intraluminal suture. Comparisons of regional cerebral blood flow figures taken throughout the experiment and lesion volume figures taken at 24 h after occlusion were made between the two groups. Total lesion volume in the SHR group was 263.6 ± 30.5 mm3 (mean ± SO), significantly larger and less variable than that in the SO group (145.4 ± 123.7mm3, p < 0.02). Throughout the ischemic period there was a tendency for rCBF to be lower in the 5HR group than in the SO group. In some animals of both groups, recirculation could not be produced by withdrawal of the suture from the ICA. The mortality rate was 9% in the SO group and 17% in the 5HR group. Intraluminal MeA occlusion in 5HRs is associated with a more consistent, reliable and reproducible volume of ischemic injury. In the studies of temporary focal cerebral ischemia, this model must be accompanied by the monitoring of regional cerebral blood flow so as to obtain more reliable results. [Neural Res 1998; 20: 265-270]

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