Uptake of D‐serine by synaptosomal P2 fraction isolated from rat brain
- 16 August 2001
- Vol. 42 (2) , 84-86
- https://doi.org/10.1002/syn.1103
Abstract
We have demonstrated that the crude synaptosome P2 fraction prepared from the rat brain is able to accumulate [3H]D‐serine in a saturable, temperature‐dependent and partially sodium‐ and potassium‐dependent manner with an affinity of a hundred micromolar range. The inhibition profile of D‐serine accumulation by various amino acids is different from those of uptake systems reported for glycine and other amino acids. The present data suggest that the endogenous D‐serine may be taken up mainly through a carrier‐mediated transport system to regulate its extracellular concentration in the mammalian brain. Synapse 42:84–86, 2001.Keywords
This publication has 14 references indexed in Scilit:
- Identification and Characterization of a Na+-independent Neutral Amino Acid Transporter That Associates with the 4F2 Heavy Chain and Exhibits Substrate Selectivity for Small Neutral d- and l-Amino AcidsJournal of Biological Chemistry, 2000
- Modulation of a Recombinant Glycine Transporter (GLYT1b) by Activation of Protein Kinase CJournal of Neurochemistry, 1995
- Glial uptake of intracerebroventricularly injected d-serine in the rat brain: an immunocytochemical studyNeuroscience Letters, 1995
- Endogenous d‐Serine in Rat Brain: N‐Methyl‐d‐Aspartate Receptor‐Related Distribution and AgingJournal of Neurochemistry, 1993
- The presence of free D‐serine in rat brainFEBS Letters, 1992
- High‐Affinity Transport of γ‐Aminobutyric Acid, Glycine, Taurine, L‐Aspartic Acid, and L‐Glutamic Acid in Synaptosomal (P2) Tissue: A Kinetic and Substrate Specificity AnalysisJournal of Neurochemistry, 1987
- Increased [3H]kainic acid binding in the prefrontal cortex in schizophreniaNeuroscience Letters, 1983
- Uptake ofl-alanine, glycine andl-serine in the pigeon central nervous systemBrain Research, 1978
- High affinity uptake systems for glycine, glutamic and aspartic acids in synaptosomes of rat central nervous tissuesBrain Research, 1972
- D-Amino Acids in AnimalsScience, 1969