Abstract
Severe autolysis may occur in bulk‐stored raw material used for the production of fish meal and oil, especially when the content of feed in the gut is high. The autolysis was mainly due to solubilisation of muscle protein caused by proteolytic enzymes which leaked from the intestinal tract. These enzymes were of the trypsin type and accounted for about 70% of the total proteolytic activity in capelin with low gut content (5 % of total fish weight). Enzymes from stomach and intestines were of about equal importance. Enzymes located in the muscle, kidney or gonads made only a minor contribution to the total autolysis. Higher gut content (11% of total fish weight) increased the proteolytic activity in the stomach and intestines and the autolysis of the capelin. Microbial proteolytic enzymes accounted for 20‐30 % of the total protein solubilisation.
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