Radiation Indication of Subunit Activity of Lactic Dehydrogenase

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Abstract
Lactic dehydrogenases extracted from various tissues of rat and goldfish were irradiated in dry films in vacuum with fast electrons. The material was redissolved, assayed for enzymatic activity remaining, and the results interpreted according to single-hit theory. For all sources tested the target mass is 30,000 to 45,000 gm/mole, suggesting that enzyme subunits demonstrable electrophoretically in this size range can bind and catalyze independently within the tetrameric complexes of the holoenzyme.